VIH Antibody Yihuta Kwipimisha Cassette Kwipimisha Indwara
VIH Antibody Yihuta Yipimisha Kit Kwipimisha Indwara
INCAMAKE
Uburyo rusange bwo kumenya ubwandu bwa virusi itera sida ni ukureba niba antibodiyite zanduye virusi hakoreshejwe uburyo bwa EIA hagakurikiraho kwemezwa na Western Blot.Kwipimisha Intambwe imwe ya virusi itera SIDA ni ikizamini cyoroshye, kigaragara cyerekana antibodies mumaraso Yumuntu Yuzuye / serum / plasma.Ikizamini gishingiye kuri immunochromatografiya kandi irashobora gutanga ibisubizo muminota 15.
UKORESHEJWE
Ikizamini kimwe cya virusi itera sida ni Zahabu ya Colloidal yongerewe imbaraga, yihuta ya Immunochromatoraphic Assay kugirango hamenyekane neza antibodi zanduye (VIH) mumaraso Yumuntu Yuzuye / Serumu / Plasma.Iki kizamini nikizamini cyo gusuzuma kandi ibyiza byose bigomba kwemezwa ukoresheje ikindi kizamini nka Western Blot.Ikizamini kigenewe ubuvuzi bukoreshwa gusa.Ikizamini hamwe n'ibisubizo by'ibizamini bigenewe gukoreshwa ninzobere mu buvuzi n’amategeko gusa, keretse iyo byemewe ukundi n’amabwiriza mu gihugu gikoreshwa.Ikizamini ntigikwiye gukoreshwa hatabayeho kugenzurwa neza.
IHame RY'UBURYO
Isuzuma ritangirana nicyitegererezo cyashyizwe kumurongo neza no kongeramo icyitegererezo cyatanzwe ako kanya.VIH antigen-Colloidal Zahabu conjugate yashyizwe mu cyitegererezo ntangarugero ikora hamwe na antibody ya sida iri muri serumu cyangwa plasma, ikora antibody ya conjugate / VIH.Nkuko uruvange rwemerewe kwimuka kuruhande rwibizamini, ikigo cya antibody ya conjugate / virusi itera sida gifatwa na poroteyine ihuza antibody A yimuwe kuri membrane ikora umurongo wamabara mukarere kizamini.Icyitegererezo kibi ntigitanga umurongo wikizamini bitewe no kubura kwa colloidal Gold conjugate / virusi itera sida.Antigene zikoreshwa mu kizamini ni poroteyine za recombinant zihuye n'uturere twa virusi itera SIDA.Itsinda ryamabara yo kugenzura mukarere kagenzura kugaragara kurangiza inzira yikizamini utitaye kubisubizo byikizamini.Iri tsinda rishinzwe kugenzura ni ibisubizo bya Colloidal Gold conjugate ihuza antibody yo kurwanya virusi itera sida yanduye kuri membrane.Umurongo wo kugenzura werekana ko Colloidal Zahabu conjugate ikora.Kubura kugenzura kugenzura byerekana ko ikizamini kitemewe.
REAGENTS N'IBIKORWA BYUZUWE
Igikoresho cyo kwipimisha kugiti cyawe gifatanye na desiccant
• Igitonyanga cya plastiki.
• Icyitegererezo
• Shyiramo
IBIKORWA BISABWA ARIKO NTIBITANZWE
Igenzura ryiza kandi ribi (riraboneka nkikintu gitandukanye)
Ububiko & STABILITY
Ibikoresho byo kwipimisha bigomba kubikwa kuri 2-30 ℃ mumufuka ufunze kandi mubihe byumye.
UMUBURO N'UBWITONDERWA
1) Ibisubizo byiza byose bigomba kwemezwa nubundi buryo.
2) Fata ingero zose nkaho zishobora kwandura.Wambare uturindantoki n'imyambaro ikingira mugihe ukoresha ingero.
3) Ibikoresho bikoreshwa mugupimisha bigomba kuba autoclave mbere yo kujugunywa.
4) Ntukoreshe ibikoresho bya kit birenze amatariki yabyo.
5) Ntugahindurane reagent kuva mubufindo butandukanye.
GUKORANYA URUGERO N'UBubiko
1) Kusanya Amaraso Yuzuye / Serumu / Plasma ikurikiza uburyo bwa laboratoire isanzwe.
2) Ububiko: Amaraso Yose ntashobora gukonjeshwa.Icyitegererezo kigomba gukonjeshwa niba kidakoreshejwe umunsi umwe wo gukusanya.Ibigereranyo bigomba guhagarikwa niba bidakoreshejwe muminsi 3 yo gukusanya.Irinde gukonjesha no gukonjesha ingero inshuro zirenga 2-3 mbere yo gukoresha.0.1% ya Sodium Azide irashobora kongerwaho kubigereranyo nkuburinzi bitagize ingaruka kubisubizo byubushakashatsi.
GUKORA UBURYO
1) Ukoresheje igitonyanga cya pulasitike gifunze kuri sample, tanga igitonyanga 1 (10μl) cyamaraso Yuzuye / Serumu / Plasma kurugero ruzenguruka rwikarita yikizamini
2) Ongeraho ibitonyanga 2 byicyitegererezo Diluent kurugero rwiza, ako kanya nyuma yikigereranyo cyongeweho, uhereye kumutwe wibitonyanga diluent vial (cyangwa ibikubiye muri ampule imwe yikizamini).
3) Sobanura ibisubizo by'ibizamini mu minota 15.
Inyandiko:
1) Gukoresha urugero ruhagije rwicyitegererezo ni ngombwa kubisubizo byemewe.Niba kwimuka (guhanagura kwa membrane) bitagaragaye mumadirishya yikizamini nyuma yiminota umwe, ongeramo ikindi gitonyanga cya diluent kurugero rwiza.
2) Ibisubizo byiza bishobora kugaragara mugihe cyumunota umwe kuburugero rufite antibodiyite nyinshi za sida.
3) Ntugasobanure ibisubizo nyuma yiminota 20
GUSOMA IBISUBIZO BYIZA
1)Ibyiza: Byombi ibara ritukura ryipimisha ritukura hamwe na bande itukura itukura igaragara kuri membrane.Hasi ya antibody yibanze, intege nke zo kwipimisha.
2) Ibibi: Gusa ibara ritukura ryumutuku rigaragara kuri membrane.Kubura itsinda ryikizamini byerekana ibisubizo bibi.
3)Ibisubizo bitemewe:Buri gihe hagomba kubaho ibara ritukura ryumutuku mugace kayobora, utitaye kubisubizo byikizamini.Niba itsinda rishinzwe kugenzura ritagaragara, ikizamini gifatwa nkicyemewe.Subiramo ikizamini ukoresheje igikoresho gishya.
Icyitonderwa: Nibisanzwe kugira bande yoroheje yoroheje igenzura hamwe nicyitegererezo cyiza cyane, mugihe cyose igaragara neza.
LIMITATION
1) Gusa bisobanutse, bishya, bitemba byubusa Amaraso Yuzuye / Serumu / Plasma arashobora gukoreshwa muriki kizamini.
2) Ibyitegererezo bishya nibyiza ariko ingero zahagaritswe zirashobora gukoreshwa.Niba icyitegererezo cyahagaritswe, kigomba kwemererwa gukonjeshwa mumwanya uhagaze kandi kigasuzumwa neza.Amaraso Yose ntashobora gukonjeshwa.
3) Ntugahungabanye icyitegererezo.Shyiramo umuyoboro munsi yubuso bwikitegererezo kugirango ukusanyirize hamwe.
