Abashakashatsi b'Abaholandi bahuza CRISPR na bioluminescence mu kizamini cyo kugeragezaindwara zanduza

Abashakashatsi bo mu Buholandi bavuga ko poroteyine nshya yakozwe nijoro ishobora kwihuta no koroshya gusuzuma indwara ziterwa na virusi.
Ubushakashatsi bwabo bwatangajwe ku wa gatatu mu gitabo cya ACS, busobanura uburyo bworoshye, intambwe imwe yo gusesengura byihuse aside nucleic acide ya virusi ndetse n’imiterere yabyo ukoresheje poroteyine zijimye cyangwa ubururu.
Kumenya indwara ziterwa na virusi mu gutunga urutoki rwa acide nucleic ni ingamba zingenzi mu gusuzuma amavuriro, ubushakashatsi ku binyabuzima, no gukurikirana ibiribwa no kubungabunga ibidukikije.Ibizamini bikoreshwa cyane muburyo bwa polymerase byerekana (PCR) biroroshye cyane, ariko bisaba gutegura icyitegererezo cyihariye cyangwa gusobanura ibisubizo, bigatuma bidashoboka kubice bimwe na bimwe byubuzima cyangwa ahantu hafite amikoro make.
Iri tsinda ryaturutse mu Buholandi ni ibisubizo by’ubufatanye hagati y’abahanga bo muri za kaminuza n’ibitaro hagamijwe guteza imbere uburyo bwihuse, bworoshye kandi bworoshye-bwo gukoresha aside nucleic aside yo kwisuzumisha ishobora gukoreshwa ahantu hatandukanye.
Bahumekewe no gucana umuriro, gucana umuriro, hamwe n'inyenyeri ntoya ya phytoplankton yo mu mazi, byose bikoreshwa na phenomenon yitwa bioluminescence.Izi ngaruka-zijimye-umwijima ziterwa na reaction ya chimique irimo proteine ​​ya luciferase.Abashakashatsi binjije poroteyine za luciferase mu byuma bitanga ibyuma bitanga urumuri kugira ngo byoroherezwe kwitegereza iyo babonye intego.Mugihe ibi bituma ibyo byuma bifata neza kugirango bimenyekanishe-kuri-kubitaho, kuri ubu ntibabura sensibilité ikenewe mugupimisha kwisuzumisha kwa muganga.Mugihe uburyo bwo guhindura gene CRISPR bushobora gutanga ubu bushobozi, busaba intambwe nyinshi nibindi bikoresho byihariye kugirango tumenye ibimenyetso bidakomeye bishobora kugaragara mubitegererezo bigoye, byuzuye urusaku.
Abashakashatsi babonye uburyo bwo guhuza poroteyine ijyanye na CRISPR hamwe na signal ya bioluminescent ishobora kugaragara hamwe na kamera yoroshye ya digitale.Kugirango hamenyekane neza ko hari urugero rwa RNA cyangwa ADN ihagije yo gusesengura, abashakashatsi bakoze recombinase polymerase amplification (RPA), tekinike yoroshye ikora ku bushyuhe buhoraho bwa 100 ° F.Bateguye urubuga rushya rwitwa Luminescent Nucleic Acide Sensor (LUNAS), aho poroteyine ebyiri za CRISPR / Cas9 zihariye ku bice bitandukanye bihuza genome ya virusi, buri kimwe gifite agace kihariye ka luciferase kiyometse hejuru.
Iyo genome yihariye ya virusi abashakashatsi barimo gusuzuma irahari, poroteyine ebyiri CRISPR / Cas9 zihuza intego ya acide nucleic;zihinduka hafi, zemerera poroteyine ya luciferase idashobora gukora no gusohora urumuri rwubururu imbere ya substrate yimiti..Kugirango babaze substrate yakoreshejwe muriki gikorwa, abashakashatsi bakoresheje uburyo bwo kugenzura butanga urumuri rwatsi.Umuyoboro uhindura ibara kuva icyatsi ujya mubururu byerekana igisubizo cyiza.
Abashakashatsi bagerageje urubuga rwabo bategura RPA-LUNAS, ibonaSARS-CoV-2 RNAhatabayeho kurambirwa RNA kwigunga, kandi yerekanaga imikorere yayo yo gusuzuma kuri nasopharyngeal swab sample kuvaCOVID-19abarwayi.RPA-LUNAS yatahuye neza SARS-CoV-2 mu minota 20 mu byitegererezo bifite umutwaro wa virusi ya RNA uri munsi ya 200 kopi / μL.
Abashakashatsi bemeza ko ubushakashatsi bwabo bushobora kumenya mu buryo bworoshye kandi neza izindi virusi nyinshi.Baranditse bati: "RPA-LUNAS irashimishije mu gupima indwara zanduza."