INCAMAKE

Uburyo rusange bwo kumenya kwandura HCV ni ukureba niba antibodies zandura virusi hakoreshejwe uburyo bwa EIA hakurikiraho kwemezwa na Western Blot.Ikizamini Cyintambwe imwe ya HCV nikizamini cyoroshye, kigaragara cyujuje ubuziranenge cyerekana antibodies mumaraso Yumuntu Yuzuye / serumu / plasma.Ikizamini gishingiye kuri immunochromatografiya kandi irashobora gutanga ibisubizo muminota 15.

UKORESHEJWE

Ikizamini Cyintambwe imwe ya HCV ni Zahabu ya Colloidal yazamuye, yihuta ya Immunochromatoraphic Assay kugirango hamenyekane neza antibodi zanduye virusi ya Hepatite C (HCV) mumaraso Yumuntu Yuzuye / Serumu / Plasma.Iki kizamini nikizamini cyo gusuzuma kandi ibyiza byose bigomba kwemezwa ukoresheje ikindi kizamini nka Western Blot.Ikizamini kigenewe ubuvuzi bukoreshwa gusa.Ikizamini hamwe n'ibisubizo by'ibizamini bigenewe gukoreshwa ninzobere mu buvuzi n’amategeko gusa, keretse iyo byemewe ukundi n’amabwiriza mu gihugu gikoreshwa.Ikizamini ntigikwiye gukoreshwa hatabayeho kugenzurwa neza.

IHame RY'UBURYO

Isuzuma ritangirana nicyitegererezo cyashyizwe kumurongo neza no kongeramo icyitegererezo cyatanzwe ako kanya.HCV antigen-Colloidal Zahabu conjugate yashyizwe mu cyitegererezo ntangarugero ikora hamwe na antibody ya HCV iboneka muri serumu cyangwa plasma, ikora antibody ya conjugate / HCV.Nkuko uruvange rwemerewe kwimuka kuruhande rwibizamini, uruganda rwa antibody ya conjugate / HCV rufatwa na poroteyine ihuza antibody A yimuwe kuri membrane ikora umurongo wamabara mukarere kizamini.Icyitegererezo kibi ntabwo gitanga umurongo wikizamini kubera ko nta antibody ya Colloidal Gold conjugate / HCV.Antigene zikoreshwa mugupimisha ni proteine ​​za recombinant zihuye n'uturere dukingira cyane HCV.Itsinda ryamabara yo kugenzura mukarere kagenzura kugaragara kurangiza inzira yikizamini utitaye kubisubizo byikizamini.Iri tsinda rishinzwe kugenzura ni ibisubizo bya Colloidal Gold conjugate ihuza antibody yo kurwanya anti-HCV yimuwe kuri membrane.Umurongo wo kugenzura werekana ko Colloidal Zahabu conjugate ikora.Kubura kugenzura kugenzura byerekana ko ikizamini kitemewe.

REAGENTS N'IBIKORWA BYUZUWE

Igikoresho cyo kwipimisha kugiti cyawe gifatanye na desiccant

• Igitonyanga cya plastiki.

• Icyitegererezo

• Shyiramo

IBIKORWA BISABWA ARIKO NTIBITANZWE

Igenzura ryiza kandi ribi (riraboneka nkikintu gitandukanye)

Ububiko & STABILITY

Ibikoresho byo kwipimisha bigomba kubikwa kuri 2-30 ℃ mumufuka ufunze kandi mubihe byumye.

UMUBURO N'UBWITONDERWA

1) Ibisubizo byiza byose bigomba kwemezwa nubundi buryo.

2) Fata ingero zose nkaho zishobora kwandura.Wambare uturindantoki n'imyambaro ikingira mugihe ukoresha ingero.

3) Ibikoresho bikoreshwa mugupimisha bigomba kuba autoclave mbere yo kujugunywa.

4) Ntukoreshe ibikoresho bya kit birenze amatariki yabyo.

5) Ntugahindurane reagent kuva mubufindo butandukanye.

GUKORANYA URUGERO N'UBubiko

1) Kusanya Amaraso Yuzuye / Serumu / Plasma ikurikiza uburyo bwa laboratoire isanzwe.

2) Ububiko: Amaraso Yose ntashobora gukonjeshwa.Icyitegererezo kigomba gukonjeshwa niba kidakoreshejwe umunsi umwe wo gukusanya.Ibigereranyo bigomba guhagarikwa niba bidakoreshejwe muminsi 3 yo gukusanya.Irinde gukonjesha no gukonjesha ingero inshuro zirenga 2-3 mbere yo gukoresha.0.1% ya Sodium Azide irashobora kongerwaho kubigereranyo nkuburinzi bitagize ingaruka kubisubizo byubushakashatsi.

GUKORA UBURYO

1) Ukoresheje igitonyanga cya pulasitike gifunze kuri sample, tanga igitonyanga 1 (10μl) cyamaraso Yuzuye / Serumu / Plasma kurugero ruzenguruka rwikarita yikizamini

2) Ongeraho ibitonyanga 2 byicyitegererezo Diluent kurugero rwiza, ako kanya nyuma yikigereranyo cyongeweho, uhereye kumutwe wibitonyanga diluent vial (cyangwa ibikubiye muri ampule imwe yikizamini).

3) Sobanura ibisubizo by'ibizamini mu minota 15.