Antibody ya Syphilis yihuta Ikizamini (Serum / Plasma / Amaraso Yose)
TP Indwara Yandurira mu mibonano mpuzabitsina Syphilis Intambwe imwe yo Kwipimisha
INCAMAKE
Uburyo rusange bwo kumenya kwandura TP bukoreshwa muri vitro qualitifique yo kumenya Syphilis (TP) Antibody mumaraso yumuntu yose, serumu cyangwa plasma. Ikizamini gishingiye.Uburyo bwa zahabukandi irashobora gutanga ibisubizo muminota 15.
UKORESHEJWE
Intambwe imwe ya TP Ikizamini ni Zahabu ya Colloidal yazamuye,.Mubuvuzi, iki gicuruzwa gikoreshwa cyane mugupima ubufasha bwindwara ya Treponema pallidum.Iki gicuruzwa ni icy'abaganga bakoresha gusa.
IHame RY'UBURYO
Igicuruzwa gikurikiza ihame rya kabiri antigen sandwich.Iyo icyitegererezo kirimo antibody ya Treponema pallidum, antibody ya Treponema pallidum muri sample ikora hamwe na zahabu ya colloidal yanditseho-syphilis recombinant antigen 1 kuri padi kugirango ibe igizwe na antigen-antibody.Urusobekerane rwa chromato rwafashwe imbere binyuze mubikorwa bya capillary kandi rufatwa na recombinant syphilis antigen 2 yashizwe kumurongo wa detecticn (T umurongo) wa nitrocellulose, hanyuma hagaragara umurongo utukura.Urusobekerane rukomeje gushushanywa hejuru, kandi ikimenyetso cyinkoko lgY colloidal ikimenyetso cya zahabu gifatwa nihene irwanya inkoko lgY antibody yubatswe ahantu hagenzurwa ubuziranenge (C umurongo) wa nitrocellulose, hanyuma hagaragara umurongo utukura.Iyo ibikubiye muri analyte murugero biri munsi yumubare muto ntarengwa wo kumenya, agace kerekana (T umurongo) ntikura ibara.
INGINGO Z'INGENZI
1.Ikizamini gipima, gipakiye kugiti cyawe mumufuka wa aluminium (1piece / umufuka, 1/5/10/25/50Igice (s) / kit)
2.Icyatsi cya plastiki gishobora gukoreshwa (1picce / igikapu, 1/5/10/25/50 igice (s) / kit)
3.Umufuka wimyanda yubuvuzi (1picce / igikapu, 1/5/10/25/50 igice (s) / kit)
4.Igitabo cy'amabwiriza (1copy / igikapu, kopi 1 / kit)
Icyitonderwa: Ibigize mubikoresho byimibare itandukanye ntabwo bihinduka.
IBIKURIKIRA
Urugero rwicyitegererezo (igice 1 / igikapu, 1/5/10/25/50 igice (s) / kit)
Pad ipamba ya alcool (igice / umufuka, 1/5/10/25/50 igice (s) / kit)
Le Urushinge rwo gukusanya amaraso (igice 1 / igikapu, 1/5/10/25/50 igice (s) / kit)
IBIKORWA BISABWA ARIKO NTIBITANZWE
Igenzura ryiza kandi ribi (riraboneka nkikintu gitandukanye)
Ububiko & STABILITY
Ipaki yumwimerere igomba kubikwa ahantu humye kuri 4-30 ℃ ikingiwe urumuri, kandi ntugahagarike.
UMUBURO N'UBWITONDERWA
Igikoresho gihamye mugihe cyigihe cyo kurangiriraho cyanditse kuri label.Ikizamini cyipimisha kigomba gukoreshwa mugihe cyisaha ya 4-30 ℃ nubushuhe <65% nyuma yumufuka wa aluminium foil umaze gupakururwa.Birasabwa kuyikoresha ako kanya mugihe cy'ubushyuhe bwinshi cyangwa ubushyuhe bwinshi.
GUKORANYA URUGERO N'UBubiko
2. Ububiko bw'icyitegererezo
2.1 Amaraso yose;Imiyoboro ya Anticoagulant ikoreshwa mugukusanya amaraso, kandi bisanzweanticoagulants irashobora gukoreshwa;niba amaraso yose adashobora gukoreshwa ako kanya nyumagukusanya, birashobora kubikwa kuri 2-8 ° C muminsi 3, kandi ibyitegererezo ntibishobora gukonjeshwa.
2.2 Serumu / plasma: Icyitegererezo gishobora kubikwa kuri 2-8 ℃ iminsi 7, kandi igomba kubabibitswe kuri -20 ℃ kubikwa igihe kirekire.
3.Gukoresha gusa ingero zidafite hemolyzed.Icyitegererezo gikwiye cyanegusubirwamo.
Ingero za firigo zigomba gusubizwa ubushyuhe bwicyumba mbere yikizamini.UwitekaIngero zafunzwe zigomba gukonjeshwa rwose, gusubirwamo, no kuvangwa neza mbereKoresha.Ntugahagarike kandi ushonje inshuro nyinshi
GUKORA UBURYO
1) Ukoresheje igitonyanga cya pulasitike gifunze kuri sample, tanga igitonyanga 1 (10μl) cyamaraso Yuzuye / Serumu / Plasma kurugero ruzenguruka rwikarita yikizamini
2) Ongeraho ibitonyanga 2 byicyitegererezo Diluent kurugero rwiza, ako kanya nyuma yikigereranyo cyongeweho, uhereye kumutwe wibitonyanga diluent vial (cyangwa ibikubiye muri ampule imwe yikizamini).
3) Sobanura ibisubizo by'ibizamini mu minota 15.
Inyandiko:
1) Gukoresha urugero ruhagije rwicyitegererezo ni ngombwa kubisubizo byemewe.Niba kwimuka (guhanagura kwa membrane) bitagaragaye mumadirishya yikizamini nyuma yiminota umwe, ongeramo ikindi gitonyanga cya diluent kurugero rwiza.
2) Ibisubizo byiza birashobora kugaragara nkumunota umwe kuburugero hamwe na antibodi nyinshi za TP.
3) Ntugasobanure ibisubizo nyuma yiminota 20
GUSOMA IBISUBIZO BYIZA
1)Ibyiza: Byombi ibara ritukura ryipimisha ritukura hamwe na bande itukura itukura igaragara kuri membrane.Hasi ya antibody yibanze, intege nke zo kwipimisha.
2) Ibibi: Gusa ibara ritukura ryumutuku rigaragara kuri membrane.Kubura itsinda ryikizamini byerekana ibisubizo bibi.
3)Ibisubizo bitemewe:Buri gihe hagomba kubaho ibara ritukura ryumutuku mugace kayobora, utitaye kubisubizo byikizamini.Niba itsinda rishinzwe kugenzura ritagaragara, ikizamini gifatwa nkicyemewe.Subiramo ikizamini ukoresheje igikoresho gishya.
Icyitonderwa: Nibisanzwe kugira bande yoroheje yoroheje igenzura hamwe nicyitegererezo cyiza cyane, mugihe cyose igaragara neza.
LIMITATION
1) Gusa bisobanutse, bishya, bitemba byubusa Amaraso Yuzuye / Serumu / Plasma arashobora gukoreshwa muriki kizamini.
2) Ibyitegererezo bishya nibyiza ariko ingero zahagaritswe zirashobora gukoreshwa.Niba icyitegererezo cyahagaritswe, kigomba kwemererwa gukonjeshwa mumwanya uhagaze kandi kigasuzumwa neza.Amaraso Yose ntashobora gukonjeshwa.
3) Ntugahungabanye icyitegererezo.Shyiramo umuyoboro munsi yubuso bwikitegererezo kugirango ukusanyirize hamwe.
