[UKORESHEJWE]

Dengue IgG / IgM Ikizamini Cassette yihuta ni chromatog- raphic immunoassay ikurikira kugirango hamenyekane neza antibodies (IgG na IgM) zanduza virusi ya dengue mumaraso Yumuntu Yuzuye / Serumu / Plasma.Itanga ubufasha mugupima kwandura virusi ya Dengue.

[INCAMAKE]

Indwara ya Dengue ni indwara yanduye iterwa na virusi iterwa na virusi ya dengue yanduzwa n'imibu.Indwara ya virusi ya Dengue irashobora gutera indwara zidakira, umuriro wa dengue, umuriro wa dengue hemorhagie, umuriro wa dengue hemorhagie.Indwara zisanzwe zigaragaza indwara ya dengue zirimo gutangira gitunguranye, umuriro mwinshi, kubabara umutwe, imitsi ikabije, amagufwa hamwe nububabare bufatanye, kurwara uruhu, kuva amaraso, kwaguka kwa lymph node, kugabanuka kwingirangingo zamaraso yera, trombocytopenia nibindi mubarwayi bamwe.Iyi ndwara ahanini iri mu turere dushyuha no mu turere dushyuha, kubera ko iyi ndwara yanduzwa n'umubu wa Aides, impamvu izwi cyane mu bihe runaka, iba muri buri mwaka ubusanzwe muri Gicurasi ~ Ugushyingo, impinga ni muri Nyakanga ~ Nzeri.Mu gace gashya k'ibyorezo, muri rusange abaturage bakunze kwibasirwa, ariko ubwandu bukaba ahanini bukuze, mu gace k’icyorezo, indwara ahanini ni abana.

[INGINGO]

Dengue IgG / IgM Rapid Test Cassette ni umurongo wujuje ubuziranenge ushingiye ku immunoassay kugirango hamenyekane antibodiyite za virusi ya dengue (IgG na IgM) mu maraso Yuzuye / Serumu / Plasma.Cassette yikizamini igizwe na: 1) padi yamabara ya burugundy irimo ibara rya dengue recombinant envelope antigens yahujwe na zahabu ya Colloid (dengue conjugates), 2) agace ka nitrocellulose karimo imirongo ibiri yikizamini (umurongo wa IgG na IgM) n'umurongo ugenzura (C umurongo ).Umurongo wa IgM wabanje gushyirwaho antibody ya Mouse irwanya Umuntu IgM, umurongo wa IgG ushyizwemo na antibody ya Mouse anti-Human IgG.Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgM anti-dengue niba ihari murugero izahuza na dengue conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yometse kumurongo wa IgM, ikora umurongo wa IgM ufite amabara ya burgundy, byerekana igisubizo cyiza cya dengue IgM kandi byerekana ko wanduye.IgG anti-dengue, niba ihari murugero, izahuza na dengue conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gutwikirwa ku gice cya IgG, igakora umurongo w'amabara ya IgG, ugaragaza igisubizo cyiza cya dengue IgG kandi kigaragaza ko wanduye vuba cyangwa ukongera.Kubura imirongo iyo ari yo yose T (IgG na IgM) byerekana ibisubizo bibi.Kugirango ukore igenzura, umurongo wamabara uzahora ugaragara mukarere kayobora umurongo werekana ko ingano ikwiye yikigereranyo yongewemo kandi gukuramo membrane byabayeho.

[Ububiko N'UBUHAMYA]

Ubike nkuko bipakiye mumufuka ufunze mubushyuhe (4-30 ℃ cyangwa 40-86 ℉).Igikoresho gihamye mugihe cyigihe cyo kurangiriraho cyanditse kuri label.

Umaze gufungura umufuka, ikizamini kigomba gukoreshwa mugihe cyisaha imwe.Kumara igihe kinini ibidukikije bishyushye nubushuhe bizatera ibicuruzwa kwangirika.

LOT n'itariki yo kurangiriraho byacapishijwe kuri label.

[UMUVUGIZI]

Ikizamini kirashobora gukoreshwa mugupima Amaraso Yuzuye / Serumu / Plasma.

Gukusanya amaraso yose, serumu cyangwa plasma ikurikiza uburyo busanzwe bwa laboratoire.

Tandukanya serumu cyangwa plasma mumaraso byihuse kugirango wirinde hemolytic.Koresha gusa ingero zidasenyutse.

Bika ingero kuri 2-8 ℃ (36-46 ℉) niba bitageragejwe ako kanya.Bika ingero kuri 2-8 ℃ kugeza kumunsi 7.Ingero zigomba gukonjeshwa kuri

-20 ℃ (-4 ℉) kubikwa igihe kirekire.Ntugahagarike urugero rwamaraso yose.

Irinde kuzenguruka inshuro nyinshi.Mbere yo kwipimisha, zana ingero zikonje mubushyuhe bwicyumba hanyuma uvange witonze.Ibigereranyo birimo ibintu bigaragara bigomba gusobanurwa na centrifugation mbere yo kwipimisha.

Ntukoreshe ingero zerekana lineman nini, hemolitike ikabije cyangwa ihungabana kugirango wirinde kwivanga mubisobanuro.

UBURYO BWO GUKORA IKIZAMINI]

Emera igikoresho cyikigereranyo hamwe ningero zingana nubushyuhe (15-30 ℃ cyangwa 59-86 ℉) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura.Kuraho cassette yikizamini mumufuka ufunze hanyuma uyikoreshe vuba bishoboka.

2. Shira cassette yikizamini hejuru yisuku kandi iringaniye.

3. Fata igitonyanga gihagaritse hanyuma wohereze igitonyanga 1 cyikigereranyo (hafi 10μl) kurugero rwiza (S) rwa cassette yikizamini, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.

4. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.