Gukoresha

Ikizamini cya Dengue NS1 Ag-IgG / IgM Combo ni immunoassay yihuse ya chromatografique yo kumenya neza antibodies (IgG na IgM) na virusi ya dengue NS1 antigen kuri virusi ya dengue muri Serum cyangwa Plasma kugirango ifashe mugupima virusi ya Dengue.

[INCAMAKE]

Indwara ya Dengue ni indwara yanduye iterwa na virusi iterwa na virusi ya dengue yanduzwa n'imibu.Indwara ya virusi ya Dengue irashobora gutera indwara zidakira, umuriro wa dengue, umuriro wa dengue hemorhagie, umuriro wa dengue hemorhagie.Indwara zisanzwe zigaragaza indwara ya dengue zirimo gutangira gitunguranye, umuriro mwinshi, kubabara umutwe, imitsi ikabije, amagufwa hamwe nububabare bufatanye, kurwara uruhu, kuva amaraso, kwaguka kwa lymph node, kugabanuka kwingirangingo zamaraso yera, trombocytopenia nibindi mubarwayi bamwe.Iyi ndwara ahanini iri mu turere dushyuha no mu turere dushyuha, kubera ko iyi ndwara yanduzwa n'umubu wa Aides, impamvu izwi cyane mu bihe runaka, iba muri buri mwaka ubusanzwe muri Gicurasi ~ Ugushyingo, impinga ni muri Nyakanga ~ Nzeri.Mu gace gashya k'ibyorezo, muri rusange abaturage bakunze kwibasirwa, ariko ubwandu bukaba ahanini bukuze, mu gace k’icyorezo, indwara ahanini ni abana.

Ihame

Ikizamini cya Dengue NS1 Ag-IgG / IgM Combo ni igipimo cyiza cya membrane gishingiye ku immunoassay yo kumenya antibodi za virusi ya dengue (IgG na IgM) na virusi ya dengue NS1 antigen muri Serum cyangwa Plasma.

Kubizamini bya IgG / IgM: Igikoresho cyo kwipimisha kigizwe na: 1) padi yamabara ya burgundy irimo ibara rya antengens ya dengue recombinant ihujwe na zahabu ya Colloid (dengue conjugates), 2) agace ka nitrocellulose karimo imirongo ibiri yikizamini (T1 na T2) na umurongo wo kugenzura (C umurongo).Umurongo wa T1 wateguwe mbere na antibody kugirango hamenyekane anti-dengue ya IgM, umurongo wa T2 wasizwe na antibody kugirango hamenyekane IgG anti-dengue.Iyo ingano ihagije yikigereranyo yatanzwe mu iriba ryikitegererezo cassette yikigereranyo, icyitegererezo cyimuka kubikorwa bya capillary hakurya ya cassette.IgG anti-dengue, niba ihari murugero, izahuza na dengue conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yabanje gushyirwaho umurongo wa T2, ikora umurongo wa T2 ufite amabara ya burgundy, byerekana ibisubizo byiza bya dengue IgG kandi byerekana ko wanduye vuba cyangwa usubiramo.IgM anti-dengue niba ihari murugero izahuza na dengue conjugates.Ubudahangarwa bw'umubiri burafatwa na reagent yometse kumurongo wa T1, ikora umurongo wa T1 ufite amabara ya burgundy, byerekana igisubizo cyiza cya dengue IgM kandi byerekana ko wanduye.Kubura imirongo iyo ari yo yose T (T1 na T2) byerekana ibisubizo bibi.

Kubizamini bya NS1: Muri ubu buryo bwo gukora ibizamini, antibody anti-Dengue NS1 ihagarikwa mu karere k’ibizamini bya cassette.Nyuma yikigereranyo Cyuzuye Cyamaraso / Serumu / Plasma gishyizwe murugero rwiza, gikora hamwe na anti-Dengue NS1 antibody yometseho ibice byakoreshejwe kuri padi.Uru ruvange rwimura chromatografique muburebure bwikizamini kandi rukorana na antibody anti-Dengue NS1.Niba urugero rurimo virusi ya dengue NS1 antigen, umurongo wamabara uzagaragara mukarere ka test yerekana ibisubizo byiza.Niba icyitegererezo kitarimo virusi ya dengue NS1 antigen, umurongo wamabara ntuzagaragara muri kano karere byerekana ingaruka mbi.

Kugirango ukore igenzura, umurongo wamabara uzahora ugaragara mukarere kayobora umurongo werekana ko ingano ikwiye yikigereranyo yongewemo kandi gukuramo membrane byabayeho.

Kubika no Guhagarara

Ubike nkuko bipakiye mumufuka ufunze mubushyuhe bwicyumba cyangwa firigo (4-30 ℃ cyangwa 40-86 ℉).Igikoresho cyo kwipimisha gihamye binyuze mumatariki yo kurangiriraho yacapishijwe kumufuka ufunze.

Ikizamini kigomba kuguma mu gikapu gifunze kugeza gikoreshejwe.

Icyegeranyo cyo gukusanya no kwitegura

1. Ikizamini cya Dengue NS1 Ag-IgG / IgM Combo Ikizamini gishobora gukorwa kuri Serumu cyangwa Plasma.

2. Gukusanya amaraso yose, serumu cyangwa plasma ikurikiza uburyo busanzwe bwa laboratoire.

3. Kwipimisha bigomba gukorwa ako kanya nyuma yo gukusanya ingero.Ntugasige ingero zubushyuhe bwicyumba igihe kirekire.Kubika igihe kirekire, ingero zigomba kubikwa munsi-20 ℃.

4. Zana ingero z'ubushyuhe bw'icyumba mbere yo kwipimisha.Ingero zikonje zigomba gukonjeshwa rwose no kuvangwa neza mbere yo kwipimisha.Ingero ntizigomba gukonjeshwa no gukonjeshwa inshuro nyinshi.

Uburyo bwo Kwipimisha

Emera ikizamini, ingero, buffer na / cyangwa igenzura kugera kubushyuhe bwicyumba 15-30 ℃ (59-86 ℉) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura.Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka.Shira igikoresho cyo kwipimisha hejuru kandi isukuye.

2. Kubizamini bya IgG / IgM: Fata igitonyanga uhagarike hanyuma wohereze igitonyanga 1 cyikigereranyo (hafi 10μl) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.

3. Kubizamini bya NS1: Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 8 ~ 10 bya serumu cyangwa plasma (hafi 100μl) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma utangire igihe.Reba ingero zikurikira.

4. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.

Gusobanura ibisubizo

Ibyiza:

Kuri IgG / IgM Ikizamini: Kugenzura umurongo byibuze umurongo umwe wikizamini ugaragara kuri membrane.Kugaragara kumurongo wa test ya T2 byerekana ko hariho antibodiyite ya dengue yihariye.Kugaragara kumurongo wa T1 yerekana ko hariho antibodiyite yihariye ya dengue.Niba kandi umurongo wa T1 na T2 ugaragara, byerekana ko kuba hariho dengue zombi antibodiyite IgG na IgM.Hasi ya antibody yibanze ni, intege nke ibisubizo ni.

Kubizamini bya NS1: Imirongo ibiri iragaragara.Umurongo umwe ugomba guhora ugaragara mukarere kayobora umurongo (C), undi umurongo ugaragara wamabara ugomba kugaragara mukarere kizamini.

Ibibi:

Umurongo umwe wamabara ugaragara mukarere kayobora (C) .Nta murongo ugaragara wamabara ugaragara mukarere kizamini.

Bitemewe: Umurongo wo kugenzura unanirwa kugaragara.Ingano yikigereranyo idahagije cyangwa tekinike yuburyo bukwiye nimpamvu zishoboka zo kugenzura umurongo kunanirwa.Ongera usubiremo inzira hanyuma usubiremo ikizamini ukoresheje igikoresho gishya.Niba ikibazo gikomeje, hagarika guhagarika ibikoresho byikizamini ako kanya hanyuma ubaze abakwirakwiza hafi.