Dengue Ns1 Igikoresho cyo Kwipimisha (Amaraso YuzuyeSerumPlasma)
Dengue Ns1 Igikoresho cyo Kwipimisha (Amaraso YuzuyeSerumPlasma)
[UKORESHEJWE]
Dengue NS1 Antigen Rapid Test Cassette / Strip ni immunoassay ya chromatografique ikurikira kugirango hamenyekane neza antigene virusi ya Dengue mumaraso Yumuntu / Serumu / Plasma.Itanga ubufasha mugupima kwandura virusi ya Dengue.
[INCAMAKE]
Indwara ya Dengue ni indwara yanduye iterwa na virusi iterwa na virusi ya dengue yanduzwa n'imibu.Indwara ya virusi ya Dengue irashobora gutera indwara zidakira, umuriro wa dengue, umuriro wa dengue hemorhagie, umuriro wa dengue hemorhagie.Indwara zisanzwe zigaragaza indwara ya dengue zirimo gutangira gitunguranye, umuriro mwinshi, kubabara umutwe, imitsi ikabije, amagufwa hamwe nububabare bufatanye, kurwara uruhu, kuva amaraso, kwaguka kwa lymph node, kugabanuka kwingirangingo zamaraso yera, trombocytopenia nibindi mubarwayi bamwe.Iyi ndwara ahanini iri mu turere dushyuha no mu turere dushyuha, kubera ko iyi ndwara yanduzwa n'umubu wa Aides, impamvu izwi cyane mu bihe runaka, iba muri buri mwaka ubusanzwe muri Gicurasi ~ Ugushyingo, impinga ni muri Nyakanga ~ Nzeri.Mu gace gashya k'ibyorezo, muri rusange abaturage bakunze kwibasirwa, ariko ubwandu bukaba ahanini bukuze, mu gace k’icyorezo, indwara ahanini ni abana.
[INGINGO]
Dengue NS1 Antigen Rapid Ikizamini Cassette / Strip ni immunoassay ishingiye ku ihame ryubuhanga bubiri bwa antibody-sandwich.Mugihe cyo kwipimisha, antibody anti-Dengue irahagarikwa mumurongo wikizamini cyibikoresho.Nyuma yuko Amaraso Yuzuye / Serumu / Plasma ashyizwe murugero rwiza, ikora hamwe na antibody anti-Dengue yatewe ibice byakoreshejwe kuri padi.Uru ruvange rwimura chromatografique muburebure bwikizamini kandi rukorana na antibody irwanya Dengue.Niba urugero rurimo antigen virusi ya dengue, umurongo wamabara uzagaragara mukarere ka test yerekana ibisubizo byiza.Niba icyitegererezo kitarimo antigen virusi ya dengue, umurongo wamabara ntuzagaragara muri kano karere byerekana ingaruka mbi.Kugirango ukore igenzura, umurongo wamabara uzahora ugaragara mukarere kayobora umurongo werekana ko ingano ikwiye yikigereranyo yongewemo kandi gukuramo membrane byabayeho.
[Ububiko N'UBUHAMYA]
Ubike nkuko bipakiye mumufuka ufunze mubushyuhe (4-30 ℃ cyangwa 40-86 ℉).Igikoresho gihamye mugihe cyigihe cyo kurangiriraho cyanditse kuri label.
Umaze gufungura umufuka, ikizamini kigomba gukoreshwa mugihe cyisaha imwe.Kumara igihe kinini ibidukikije bishyushye nubushuhe bizatera ibicuruzwa kwangirika.
LOT n'itariki yo kurangiriraho byacapishijwe kuri label.
[UMUVUGIZI]
Ikizamini kirashobora gukoreshwa mugupima Amaraso Yuzuye / Serumu / Plasma.
Kusanya urugero rwamaraso (arimo EDTA, citrate cyangwa heparin) ukoresheje imitsi ikurikiza uburyo busanzwe bwa laboratoire.
Tandukanya serumu cyangwa plasma mumaraso byihuse kugirango wirinde hemolytic.Koresha gusa ingero zidasenyutse.
Bika ingero kuri 2-8 ℃ (36-46 ℉) niba bitageragejwe ako kanya.Bika ingero kuri 2-8 ℃ kugeza kumunsi 7.Ingero zigomba gukonjeshwa kuri -20 ℃ (-4 ℉) kugirango zibike igihe kirekire.Ntugahagarike urugero rwamaraso yose.
Irinde kuzenguruka inshuro nyinshi.Mbere yo kwipimisha, zana ingero zikonje mubushyuhe bwicyumba hanyuma uvange witonze.Ibigereranyo birimo ibintu bigaragara bigomba gusobanurwa na centrifugation mbere yo kwipimisha.
Ntukoreshe ingero zerekana lineman nini, hemolitike ikabije cyangwa ihungabana kugirango wirinde kwivanga mubisobanuro.
UBURYO BWO GUKORA IKIZAMINI]
- Emera igikoresho cyikigereranyo hamwe ningero zingana nubushyuhe (15-30 ℃ cyangwa 59-86 ℉) mbere yo kwipimisha.
- [Kuri Strip]
1. Kuraho ikizamini cyikizamini mumufuka ufunze hanyuma ukoreshe vuba bishoboka.
2. Shira umurongo wikizamini hejuru yisuku kandi iringaniye.
3. Kubireba serumu cyangwa plasma: Fata igitonyanga uhagaritse kandi wohereze ibitonyanga 3 bya serumu cyangwa plasma (hafi 100μl) kuri padi yikigereranyo cyibizamini, hanyuma utangire igihe.Reba ingero zikurikira.
4. kubigereranyo byamaraso yose: Fata igitonyanga uhagarike hanyuma wohereze igitonyanga 1 cyamaraso yose (hafi 35μl) kuri padi yikigereranyo cyibizamini, hanyuma ongeramo ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.
5. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.
1.Kuraho cassette yikizamini mumufuka ufunze hanyuma uyikoreshe vuba bishoboka.
2. Shira cassette yikizamini hejuru yisuku kandi iringaniye.
3.Kubera serumu cyangwa plasma: Fata igitonyanga uhagarike hanyuma wohereze ibitonyanga 3 bya serumu cyangwa plasma (hafi 100μl) kurugero rwiza (S) rwa cassette yikizamini, hanyuma utangire igihe.Reba ingero zikurikira.
4.Kurugero rwamaraso yose: Fata igitonyanga uhagarike hanyuma wohereze igitonyanga 1 cyamaraso yose (hafi 35μl) kumuriba wikigereranyo (S) wa cassette yikizamini, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.
5.Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.
[GUSOBANURA IBISUBIZO]
Ibyiza: * Imirongo ibiri iragaragara.Umurongo umwe wamabara ugomba kuba mukarere kagenzura (C), naho undi murongo ugaragara wamabara yegeranye ugomba kuba mukarere kizamini (T).Igisubizo cyiza cyerekana ko hari antigene kuri Dengue.
Ibibi: Umurongo umwe wamabara ugaragara mukarere kagenzura (C).Nta murongo ugaragara mukarere k'ibizamini (T).Ibisubizo bibi byerekana kubura antigene kuri Dengue.
Bitemewe: Umurongo wo kugenzura unanirwa kugaragara.Ingano yikigereranyo idahagije cyangwa tekinike yuburyo bukwiye nimpamvu zishoboka zo kugenzura umurongo kunanirwa.Ongera usuzume inzira hanyuma usubiremo ikizamini ukoresheje cassette nshya.Niba ikibazo gikomeje, hagarika gukoresha ubufindo ako kanya hanyuma ubaze uwagukwirakwiza.
