HCV Ikizamini cyihuta (WB / S / P)
HCV Ikizamini Cyihuta Cassette / Strip / kit (WB / S / P)
[UKORESHEJWE]
Ikizamini cya HCV cyihuta Cassette / Strip ni immunoassay ikurikira ya chromatografique kugirango igaragaze neza antibodi zanduye virusi ya Hepatitis C mumaraso yose / Serumu / Plasma.Itanga ubufasha mugupima kwandura virusi ya Hepatitis C.
[INCAMAKE]
Virusi ya Hepatitis C (HCV) ni virusi imwe ya RNA yo mu muryango wa Flaviviridae kandi ni yo nyirabayazana wa Hepatite C. Hepatitis C ni indwara idakira yibasira abantu bagera kuri miliyoni 130-170 ku isi.OMS ivuga ko buri mwaka, abantu barenga 350.000 bapfa bazize indwara z'umwijima ziterwa na hepatite C naho abantu miliyoni 3-4 banduye HCV.Abagera kuri 3% by'abatuye isi bavuga ko banduye HCV.Abantu barenga 80% banduye HCV barwara indwara zumwijima zidakira, 20-30% barwara cirrhose nyuma ya 20-30 yr, naho 1-4% bapfa bazize cirrhose cyangwa kanseri yumwijima.Abantu banduye HCV bakora antibodies kuri virusi kandi kuba izo antibodi ziri mumaraso byerekana kwandura kwa HCV cyangwa kera.
[UMURIMO](25sets / 40sets / 50sets / ibisobanuro byihariye byose byemewe)
Ikizamini cassette / strip kirimo umurongo wa membrane ushyizwemo na antigen ya HCV kumurongo wikizamini, antibody yinkwavu kumurongo ugenzura, hamwe nipapuro ryirangi ririmo zahabu ya colloidal ifatanije na antombine HCV.Ingano y'ibizamini yacapishijwe kuri label.
Ibikoresho Yatanzwe
Ikizamini cassette / umurongo
Ongeramo paki
Buffer
Ibikoresho bisabwa ariko ntibitangwa
Ikigereranyo cyo gukusanya ibikoresho
Igihe
Uburyo busanzwe butananirwa gutandukanya virusi mumico ya selile cyangwa kuyishushanya na microscope ya electron.Gukwirakwiza genome ya virusi byatumye bishoboka gukora serologique ikoresha antombens recombinant.Ugereranije n'igisekuru cya mbere HCV EIAs ikoresheje antigen imwe ya recombinant, antigene nyinshi zikoresha proteine za recombinant na / cyangwa peptide ya peptide yongewemo mu bizamini bishya bya serologiya kugira ngo birinde kwambukiranya imipaka no kongera ubukana bw’ibizamini bya antibody ya HCV.HCV yihuta yipimisha Cassette / Strip itahura antibodies zandura HCV mumaraso Yuzuye / Serumu / Plasma.Ikizamini cyifashishije poroteyine A ibice bitwikiriye hamwe na poroteyine za HCV za recombinant kugirango uhitemo neza antibodi kuri HCV.Poroteyine za recombinant HCV zikoreshwa mu kizamini zashyizweho na genes zombi (nucleocapsid) na poroteyine zidafite imiterere.
[INGINGO]
Ikizamini cya HCV cyihuta Cassette / Strip ni immunoassay ishingiye ku ihame ryubuhanga bubiri bwa antigen-sandwich.Mugihe cyo kwipimisha, Amaraso Yuzuye / Serumu / Plasma urugero rwimuka hejuru kubikorwa bya capillary.Antibodies kuri HCV niba zihari murugero zizahuza na conjugate ya HCV.Ubudahangarwa bw'umubiri burafatirwa kuri membrane na antigens ya HCV yabanje gushyirwaho, hanyuma umurongo ugaragara w'amabara uzagaragara mu karere k'ibizamini byerekana ibisubizo byiza.Niba antibodies kuri HCV zidahari cyangwa zihari munsi yurwego rushobora kugaragara, umurongo wamabara ntushobora kuboneka mukarere k'ibizamini byerekana ingaruka mbi.
Kugirango ukore igenzura, umurongo wamabara uzahora ugaragara mukarere kayobora umurongo, byerekana ko ingano ikwiye yikigereranyo yongeweho kandi ibyabaye byabayeho.
(Ishusho ireba gusa, nyamuneka reba ibintu bifatika.) [Kuri Cassette]
Kuraho cassette yikizamini mumufuka ufunze.
Kuri serumu cyangwa plasma ntangarugero: Fata igitonyanga uhagarike hanyuma wohereze ibitonyanga 3 bya serumu cyangwa plasma (hafi 100μl) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma utangire igihe.Reba ingero zikurikira.
Kubigereranirizo byamaraso yose: Fata igitonyanga uhagarike hanyuma wohereze igitonyanga 1 cyamaraso yose (hafi 35μl) kumuriba wikigereranyo (S) wigikoresho cyipimishije, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.
Tegereza umurongo (amabara) ugaragara.Sobanura ibisubizo by'ibizamini mu minota 15.Ntusome ibisubizo nyuma yiminota 20.
[UMUBURO N'UBWITONDERWA]
Kuri vitro kwisuzumisha koresha gusa.
Kubashinzwe ubuzima ninzobere aho barerera.
Ntukoreshe nyuma yitariki yo kurangiriraho.
Nyamuneka soma amakuru yose muriki gitabo mbere yo gukora ikizamini.
Ikizamini cassette / strip igomba kuguma mumufuka ufunze kugeza ikoreshejwe.
Ingero zose zigomba gufatwa nkaho zishobora guteza akaga kandi zigakorwa muburyo bumwe nuwanduye.
Ikizamini cyakoreshejwe cassette / strip kigomba gutabwa ukurikije amategeko ya leta, leta ndetse n’ibanze.
[KUGENZURA UMUNTU]
Igenzura rikorwa ririmo ikizamini.Umurongo wamabara ugaragara mukarere kayobora (C) ufatwa nkigenzura ryimbere.Yemeza ingano yikigereranyo ihagije, gukuramo membrane ihagije hamwe nubuhanga bukurikirana.
Igipimo cyo kugenzura ntabwo gitangwa niki gikoresho.Ariko, birasabwa ko igenzura ryiza kandi ribi ryageragezwa nkigikorwa cyiza cya laboratoire kugirango hemezwe inzira yikizamini no kugenzura imikorere ikwiye.
[LIMITATIONS]
HCV yihuta yikizamini Cassette / Strip igarukira gutanga ibisobanuro byujuje ubuziranenge.Ubwinshi bwumurongo wikizamini ntabwo byanze bikunze bifitanye isano nubunini bwa antibody mumaraso.
Ibisubizo byabonetse muri iki kizamini bigamije kuba imfashanyo mugupima gusa.Buri muganga agomba gusobanura ibisubizo afatanije namateka yumurwayi, ibyagaragaye kumubiri, nubundi buryo bwo gusuzuma.
Igisubizo kibi cyerekana ko antibodies kuri HCV zidahari cyangwa kurwego rutamenyekana nikizamini.
[IMIKORESHEREZE Y’IMIKORESHEREZE]
Ukuri
Amasezerano hamwe nubucuruzi HCV Ikizamini cyihuse
Kugereranya kuruhande byakozwe hakoreshejwe ikizamini cyihuse cya HCV hamwe nubucuruzi bwihuse bwa HCV.Ibipimo 1035 by’amavuriro bivuye mu bitaro bitatu byasuzumwe hamwe na HCV Rapid Test hamwe nibikoresho byubucuruzi.Ingero zagenzuwe hamwe na RIBA kugirango hemezwe ko antibody ya HCV ihari.Ibisubizo bikurikira byatanzwe muri ubu bushakashatsi bwubuvuzi:
| Ubucuruzi HCV Ikizamini cyihuse | Igiteranyo | |||
| Ibyiza | Ibibi | |||
| HEO TECH® | Ibyiza | 314 | 0 | 314 |
| Ibibi | 0 | 721 | 721 | |
| Igiteranyo | 314 | 721 | 1035 | |
Amasezerano hagati yibi bikoresho byombi ni 100% kubintu byiza, na 100% kubintu bibi.Ubu bushakashatsi bwerekanye ko Ikizamini cyihuta cya HCV gihwanye cyane nibikoresho byubucuruzi.
Amasezerano na RIBA
Ibipimo 300 byamavuriro byasuzumwe hamwe na HCV Rapid Test hamwe nibikoresho bya HCV RIBA.Ibisubizo bikurikira byatanzwe muri ubu bushakashatsi bwubuvuzi:
| RIBA | Igiteranyo | |||
| Ibyiza | Ibibi | |||
| HEO TECH® | Ibyiza | 98 | 0 | 98 |
| Ibibi | 2 | 200 | 202 | |
| Igiteranyo | 100 | 200 | 300 | |
