COVID-19 Kutabogama Antibody Yihuta Ikizamini Cassette (Zahabu ya Colloidal)
COVID-19 Kutabogama Antibody Yihuta Yipimisha Cassette Gold Zahabu ya Colloidal） ni immunoassay yihuse ya chromatografique kugirango hamenyekane ubuziranenge bwa antibodiyite kuri COVID-19 mumaraso yabantu yose, serumu, cyangwa plasma nkubufasha mugupima ko hariho antibodiyite zidafite aho zibogamiye. kuri COVID-19.
Igitabo coronavirus ni icy'ubwoko.COVID-19 ni indwara yandurira mu myanya y'ubuhumekero.Abantu muri rusange birashoboka.Kugeza ubu, abarwayi banduye igitabo cyitwa coronavirus ni isoko nyamukuru yo kwandura;abantu banduye badafite ibimenyetso na bo barashobora kuba isoko yanduye.Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukuramo ni iminsi 1 kugeza 14, ahanini iminsi 3 kugeza 7.Ibyigaragaza nyamukuru birimo umuriro, umunaniro hamwe no gukorora byumye.Umubyimba wizuru, izuru ritemba, kubabara mu muhogo, myalgia na diarrhea usanga mubihe bike.
COVID-19 Kutabogama Antibody Yihuta Yipimisha Cassette Gold Zahabu ya Colloidal） ni ikizamini cyihuse gikoresha uruvange rwa S-RBD antigen yashizwemo ibara ryamabara kugirango hamenyekane antibodiyite zangiza COVID-19 mumaraso yose yumuntu, serumu, cyangwa plasma.
COVID-19 Kutabogama Antibody Yihuta Yipimisha Cassette Gold Zahabu ya Colloidal） ni membrane yujuje ubuziranenge ishingiye kuri immunoassay kugirango hamenyekane antibodiyite zangiza COVID-19 mumaraso yose, serumu cyangwa plasma.Membrane yabanje gushyirwaho na Angiotensin I Guhindura Enzyme 2 (ACE2) kumurongo wibizamini bya kariya gace.Mugihe cyo kwipimisha, amaraso yose, serumu cyangwa plasma yerekana reaction hamwe na S-RBD conjugated zahabu ya colloid.Uruvange rwimuka hejuru kuri membrane chromatografique kubikorwa bya capillary reaction kugirango ikore hamwe na ACE2 kuri membrane ikabyara umurongo wamabara.Kubaho k'uyu murongo w'amabara byerekana ibisubizo bibi, mugihe kubura kwayo byerekana ibisubizo byiza.Kugirango ube igenzura ryikurikiranabikorwa, umurongo wamabara uzahora uhinduka uva mubururu ugahinduka umutuku mukarere kayobora umurongo, byerekana ko ingano ikwiye yikigereranyo yongeweho kandi gukubita membrane byabayeho.
| Ibikoresho bipima kugiti cyawe | Buri gikoresho kirimo umurongo ufite conjugate yamabara hamwe na reagent reaction yabanje gukwirakwira mukarere kajyanye |
| Imiyoboro ikoreshwa | Kongera ingero zikoreshwa |
| Buffer | Fosifate yashizwemo saline kandi ikingira |
| Ongeramo paki | Kubikorwa byo gukora |
Ibikoresho byatanzwe
| Devices Ibikoresho byo kugerageza | Ibitonyanga |
| ● Buffer | Shyiramo paki |
Ibikoresho bisabwa ariko ntibitangwa
| Icyegeranyo cyibikoresho byo gukusanya | ● Igihe |
| Rif Centrifuge |
1. Kubanyamwuga muri vitro yo gusuzuma gusa.
2. Ntukoreshe nyuma yitariki yo kurangiriraho yerekanwe kuri paki.Ntukoreshe ikizamini niba umufuka wangiritse wangiritse.Ntukongere gukoresha ibizamini.
3. Gukuramo reagent igisubizo kirimo igisubizo cyumunyu niba igisubizo gihuza uruhu cyangwa ijisho, kwoza amazi menshi.
4. Irinde kwanduzanya kw ingero ukoresheje ibikoresho bishya byo gukusanya icyitegererezo kuri buri cyitegererezo cyabonetse.
5. Soma uburyo bwose witonze mbere yo kwipimisha.
6. Ntukarye, kunywa cyangwa kunywa itabi ahantu hakorerwa ingero n'ibikoresho.Koresha ingero zose nkaho zirimo ibintu byanduza.Kurikiza ingamba zashyizweho zo kwirinda ingaruka ziterwa na mikorobe mugihe cyose kandi ukurikize inzira zisanzwe zo guta neza ingero.Wambare imyenda ikingira nk'amakoti ya laboratoire, uturindantoki twajugunywe hamwe no kurinda amaso mugihe ingero zapimwe.
7. Niba hakekwa kwandura virusi ya coronavirus hashingiwe ku bipimo ngenderwaho byo gusuzuma amavuriro n’ibyorezo by’indwara byasabwe n’inzego z’ubuzima rusange, ingero zigomba gukusanywa hakoreshejwe uburyo bukwiye bwo kurwanya indwara zanduza virusi no koherezwa mu ishami ry’ubuzima cyangwa ry’ibanze kugira ngo ryipimishe.Umuco wa virusi ntukwiye kugeragezwa muribi bihe keretse BSL 3+ iboneka kugirango yakire hamwe numuco.
8. Ntugahindurane cyangwa kuvanga reagent kuva mubufindo butandukanye.
9. Ubushuhe n'ubushuhe birashobora kugira ingaruka mbi kubisubizo.
10. Ibikoresho byo kwipimisha byakoreshejwe bigomba gutabwa hakurikijwe amabwiriza yaho.
1. Igikoresho kigomba kubikwa kuri 2-30 ° C kugeza igihe kirangirire cyacapishijwe kumufuka ufunze.
2. Ikizamini kigomba kuguma mu gikapu gifunze kugeza gikoreshejwe.
3. Ntukonje.
4. Hagomba kwitonderwa kurinda ibice bigize kitanduye.Ntukoreshe niba hari ibimenyetso byerekana mikorobe yanduye cyangwa imvura.Kwanduza ibinyabuzima gutanga ibikoresho, kontineri cyangwa reagent bishobora kuganisha kubisubizo bitari byo.
Reba ibikoresho byose bikomoka kumuntu byanduye kandi ubikoreshe ukoresheje uburyo busanzwe bwibinyabuzima.
Amaraso Yuzuye
Karaba ikiganza cy'umurwayi hanyuma wemere gukama.Kanda ikiganza udakora kuntoki.Gutobora uruhu ukoresheje lancet sterile.Ihanagura ikimenyetso cya mbere cyamaraso.Koresha buhoro buhoro ukuboko kuva ku kuboko kugera ku kiganza kugera ku rutoki kugira ngo ukore igitonyanga cy'amaraso hejuru y'ahantu hacitse.Ongeramo Fingerstick Yuzuye Amaraso mugikoresho cyo kwipimisha ukoresheje umuyoboro wa capillary cyangwa ibitonyanga bimanikwa.
Amaraso Amaraso Yuzuye:
Kusanya urugero rwamaraso muri lavender, ubururu cyangwa icyatsi cyo hejuru cyo gukusanya (kirimo EDTA, citrate cyangwa heparin, muri Vacutainer®) ukoresheje veinpuncture.
Plasma
Kusanya urugero rwamaraso muri lavender, ubururu cyangwa icyatsi cyo hejuru cyo gukusanya (kirimo EDTA, citrate cyangwa heparin, muri Vacutainer®) ukoresheje veinpuncture.Tandukanya plasma ukoresheje centrifugation.Witonze ukure plasma mumashanyarazi mashya yabanjirije.
Serumu
Kusanya urugero rwamaraso mumiyoboro itukura yo hejuru (idafite anticoagulants muri Vacutainer®) ukoresheje veinpuncture.Emera amaraso.Tandukanya serumu ukoresheje centrifugation.Witonze ukure serumu mumashya mashya yabanjirije.
Ikigereranyo cyikigereranyo vuba bishoboka nyuma yo gukusanya.Bika ingero kuri 2 ° C-8 ° C niba bitageragejwe ako kanya.
Bika ingero kuri 2 ° C-8 ° C kugeza kuminsi 5.Ingero zigomba gukonjeshwa kuri -20 ° C kugirango zibike igihe kirekire.
Irinde kuzenguruka inshuro nyinshi.Mbere yo kwipimisha, zana ingero zikonje mubushyuhe bwicyumba hanyuma uvange witonze.Ibigereranyo birimo ibintu bigaragara bigomba gusobanurwa na centrifugation mbere yo kwipimisha.Ntugakoreshe ingero zerekana lipemia nini, hemolysis nini cyangwa imivurungano kugirango wirinde kwivanga mubisobanuro.
Zana icyitegererezo hamwe nibizamini byubushyuhe bwicyumba Kuvanga icyitegererezo neza mbere yo kwisuzumisha.Shira igikoresho cyo kwipimisha hejuru, isukuye.
Kuri capillary y'amaraso yose y'icyitegererezo:
Gukoresha umuyoboro wa capillary: Uzuza umuyoboro wa capillary naohereza hafi 50µL (cyangwa ibitonyanga 2) byintoki zamaraso yoseicyitegererezo kuri sample neza (S) yikizamini, hanyuma ongerahoIgitonyanga 1 (hafi 30 µL)ByaIcyitegererezoako kanya mucyitegererezo neza.
Amaraso yose yatanzwe:
Uzuza igitonyanga hamwe nicyitegererezo nonehokwimura ibitonyanga 2 (hafi 50 µL)by'icyitegererezo mu cyitegererezo neza.Kureba neza ko nta mwuka uhumeka.Hanyumakwimura igitonyanga 1 (hafi 30 µL)by'icyitegererezo Diluent ako kanya muri sample neza.
Kuri Plasma / Serumu icyitegererezo:
Uzuza igitonyanga hamwe nicyitegererezo nonehokwimura igitonyanga 1 (hafi 25 µL)by'icyitegererezo mu cyitegererezo neza.Kureba neza ko nta mwuka uhumeka.Hanyumakwimura igitonyanga 1 (hafi 30 µL) by'icyitegererezo Diluent ako kanya muri sample neza.
Shiraho ingengabihe.Soma ibisubizo muminota 15.Ntusome ibisubizo nyuma20 iminota.Kugira ngo wirinde urujijo, fata igikoresho cyo kwipimisha nyuma yo gusobanura ibisubizo
| UMWANZURO WA POSITIVE:
| Itsinda rimwe ryamabara gusa rigaragara mukarere kayobora (C).Nta bande y'amabara igaragara mugace kizamini (T). |
| IGISUBIZO CY'INGENZI:
| Imirongo ibiri y'amabara igaragara kuri membrane.Itsinda rimwe rigaragara mukarere kagenzura (C) naho irindi tsinda rigaragara mukarere ka test (T). |
| * ICYITONDERWA: Ubwinshi bwamabara mukarere k'ibizamini bizatandukana bitewe nubunini bwo kutabuza antibodi kuri COVID-19 murugero.Kubwibyo, igicucu icyo aricyo cyose cyamabara mukarere kizamini kigomba gufatwa nkibibi. | |
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IBISUBIZO BIDASANZWE:
| Igenzura ryananiwe kugaragara.Ibisubizo bivuye mu kizamini icyo aricyo cyose kitigeze kigenzura umurongo mugihe cyagenwe cyo gusoma kigomba gusuzugurwa.Nyamuneka suzuma inzira hanyuma usubiremo ikizamini gishya.Niba ikibazo gikomeje, hagarika gukoresha ako kanya hanyuma ubaze abaguzi baho. |
1. Igenzura ry'imbere:Iki kizamini kirimo ibintu byubatswe byubatswe, C band.Umurongo C utera imbere nyuma yo kongeramo ingero na sample diluent.Bitabaye ibyo, subiramo inzira zose hanyuma usubiremo ikizamini ukoresheje igikoresho gishya.
2. Igenzura ryo hanze:Imyitozo myiza ya Laboratoire irasaba gukoresha igenzura ryo hanze, ryiza nibibi (byatanzwe bisabwe), kugirango wizere neza imikorere.
